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How does NanoBiT compare to split firefly luciferase? The bright signal of the NanoBiT™ PPI Assay allows fusion proteins to be expressed at or near physiological levels in many cases, helping to reduce experimental artifacts. In this example, a FKBP:FRB model system is used to compare NanoBiT to a split firefly luciferase system. Both approaches gave similar EC50 values for rapamycin activation, but showed major differences in luminescence intensity. Learn more here: :http://bit.ly/1Sf3vyJ | Pro
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