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PCR Test Step by Step | Principle & Procedure
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PCR Test Step by Step | Principle & Procedure
PCR 🔹 Aim To amplify specific DNA sequences for detection or study. 🔹 Steps Denaturation: Heat DNA (~94–95°C) → separates strands. Annealing: Cool (~50–65°C) → primers bind target sequence. Extension/Elongation: DNA polymerase synthesizes new strand (~72°C). Repeat: 25–35 cycles → exponential DNA amplification. 🔹 ...
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